MONDAY 08.05 | morning | Introduction: Light, Water, Temperature, CO2: Photosynthesis Types as a flexible Response to Microclimate | |||||
Theory: Biochemistry of photosynthesis | |||||||
afternoon | Theory: Photosynthesis Metabolome | ||||||
Experimental design of the experiment, Clusia species and varieties | |||||||
Visiting the experimental locations | |||||||
TUESDAY 09.05 | morning | greenhouse facilities: microclimate, Introduction to microclimate and photosynthesis measurement Fluorescense: PEA, Imaging PAM, SPAD, Soil moisture, Porometry | |||||
afternoon | Theory: photosynthesis measurement Fluorescense: PEA, Imaging PAM, SPAD, Porometry | ||||||
WEDNESDAY 10.05 | morning | DEMO of analytical devices: GC-MS | |||||
1st harvest of Cusia – 10-11:00 = peak of CAM acidity | |||||||
afternoon | DEMO of analytical devices: KI -HPLC, Titration | ||||||
2nd harvest of Cusia – 15- 16:00 = medium of CAM acidity | |||||||
3rd harvest of Clusia – 21-22:00 = minimum of CAM acidity | |||||||
THURSDAY 11.05 | morning | Predawn measurements: PEA | |||||
4th harvest of Clusia – 05:00- 6:00 = buildup of CAM acidity | |||||||
afternoon | Sample processing in ÜR5 and Mosys Lab | ||||||
Friday 12.05 | Sample processing Analysis in Mosys Lab | ||||||
MONDAY 15.05 | morning | Plant anatomy Crosssections C3, C4, CAM | Plant anatomy Stomata Imprints C3, C4, CAM | ||||
afternoon | Plant anatomy Crosssections C3, C4, CAM | Chemistry pH | Chemistry Cation HPLC | ||||
TUESDAY 16.05 | morning | Chlorophyll content Photometer | Chemistry pH, EC, RP | ||||
afternoon | Climatic Station Microclimate Readout of data Estimation of Specific Leaf Area SLA | Data evaluation: | Porometry PEA Imaging PAM Soil moisture Chlorophyll SPAD SLA Stomata imprints Leaf temperature | ||||
WEDNESDAY 17.05 | morning | Data evaluation: | GCMS Cation HPLC Titration Chlorophyll photometer Statistics | ||||
afternoon | Data evaluation: | GCMS Cation HPLC Titration total acidity Chlorophyll photometer Statistics | |||||
THURSDAY 18.05 | morning | final statistics, graphics | |||||
afternoon | Final evaluation, prep. of protocols (3 presentations, each group) | ||||||
FRIDAY 19.05 | Reserve | Final Presentation |